Authors :
Arulraj Jeyaraj* , and Lakshmanan Nambi**
Author Address :
*Department of Botany, Cardamom Planters' Assocation College, (Affiliated to Madurai Kamaraj University, Madurai), Bodinayakanur – 625 513, Tamil Nadu. India. **Department of Botany, Vivekananda College, (Autonomous), (Affiliated to Madurai Kamaraj University, Madurai), Tiruvedakam West, Madurai, Tamil Nadu - 625234. India. E-mail : nambilaks_61@yahoo.co.in
Abstract :
The impact of Tulsi (Ocimum sanctum Linn.) plant methanolic leaf extract on the innate immunity of serum immunoglobulin levels in Tilapia Oreochromis mossambicus (Peters) are elaborately discussed here. Fishes were administrated orally 0.01, 0.1 and 1% and intraperitoneally injected with 0.32, 1.6 and 8 mg kg-1 body weight, of Tulsi leaf extract respectively. The non-specific humoral assay specifically serum immunoglobulin level was monitored. The serum immunoglobulin assay was performed at the end of every week by following three weeks feed supplement and intraperitoneal administration of Tulsi leaf extract. First week post administration of extract showed significant increase in serum immunoglobulin level on days 7, 21 and 28 at 0.1 and 0.01% (P<0.01) of the treated groups. Second weeks treatment groups showed a significant increase in immunoglobulin level on all days tested at 0.1 and 0.01% (P<0.01). Similarly, three week treatment groups of 0.1 and 1% (P < 0.01) showed a moderate increase in the serum immunoglobulin level on all treated days tested. In the intraperitoneal treatment groups were tested significantly increased the level of immunoglobulin on day 14 and 21 at 1.6 and 0.32 mg kg-1 (P<0.01). The results strongly revealed that the immunstimulatory properties of Ocimum sanctum leaf extract and it can be used as an immunostimulant in local fish farm regions.
Keywords :
Ocimum sanctum, Oreochromis mossambicus, methanolic leaf extract, serum immunoglobulin, Immunostimulant and Innate immunity.